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KMID : 0379119870150010023
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Korean Journal of Mycology
1987 Volume.15 No. 1 p.23 ~ p.28
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Progesterone Hydroxylation by Rhizopus nigricans (1)
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Abstract
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Physicochemical factors influencing the 11α-hydroxylation of progesterone by Rhizopus nigricans Ehrenberg were studied. The higher is the concentration of progesterone, the shorter time is need to attain maximum yield in the 11α-hydroxyprogesterone. High concentration in progesterone was supposed to inhibit the hydroxylation reaction of the 11α-hydroxyprogesterone. The range of optimum pH and temperature appeared to be rather broad and these conditions were similar to those of optimum growth of the mycelia. Agitation at a higher speed than a certain limit, and the presence of sugars accelerated the 11α-hydroxylation reaction. Glucose was found to be more effective for the reaction using spores rather than mycelia as enzyme sources. Organic solvents were proved to supress the enzyme activity, which decreased with increase in the concentration of the organic solvents and in the time for pretreatment with the solvents.
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